clinical approach of high technology techniques for control and elimination of endodontic microbiota

the main goal in endodontic treatment is to eradicate or at least reduce intraradicular microbial population to levels that are more compatible with periapical lesions healing process. since endodontic infections are polymicrobial in nature, intraradicular survival of endodontic microbiota and their pathogenic properties are influenced by a combination of their virulence factors. the purpose of this article is to review the endodontic microbiota and their respective virulence attributes, as well as perform a literature review of the effects of disinfection procedures in the treatment of endodontic infections to gain best practices. conventional technique for root canal preparation includes mechanical debridement and application of antimicrobial irrigants. recently, laser irradiation has been used to enhance the results of root canal treatment through its thermal effect. to reduce thermal side effects, laser activated irrigation (lai) and photon induced photoacoustic streaming (pips) were introduced. antimicrobial photodynamic therapy (apdt) by photochemical reaction uses light at a specific wavelength to activate a nontoxic photosensitizer (ps) in the presence of oxygen to produce cytotoxic products. different pss are used in dentistry including methylene blue (mb), toluidine blue o (tbo), indocyanine green (icg) and curcumin. among different options, icg could be the best choice due to its peak absorption at wavelength of 808 nm, which coincides with the commercial diode laser devices. also, this wavelength has more penetration depth compared to other wavelengths used in apdt.